Effect of lysozyme on invasion and migration of human lung carcinoma A549 cells
نویسندگان
چکیده
Objective: To investigate the effect of lysozyme (LYZ) on invasion and migration of the human lung carcinoma A549 cells. Methods: The LYZ knockdown lentivirus (LV3-LYZ) was used to silence the expression of LYZ gene. GFP fluorescence detector and Western bolting were used to detect the efficiency and effectiveness of gene silencing. Effect of LYZ expression silence on invasion ability of the A549 cells was detected by Transwell invasion assay; effect of LYZ expression silence on migration ability of the cells was detected by wound scratch assay and Transwell migration assay; effect of LYZ expression silence on cytoskeleton was detected by the phalloidin-labelled cytoskeleton assay; and effect of LYZ expression silence on the expression of RhoA and ROCK was detected by Western blotting. Results: Transfection with LV3-LYZ lentivirus could effectively inhibit the expression of LYZ protein. Silencing the expression of LYZ could inhibit invasion and migration of the human lung carcinoma A549 cells. The cytoskeleton staining results showed that compared with the LV3-NC group, F-actin staining of the A549 cells transfected with LV3-LYZ decreased significantly, and cell membrane ruffle and pseudopod formation were significantly reduced. Silencing the expression of LYZ could down-regulate the expression of RhoA and ROCK. Conclusion: Silencing the expression of LYZ inhibits invasion and migration ability of the lung carcinoma A549 cells by influencing cytoskeleton and down-regulating the expression of RhoA and ROCK.
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